Method 3640A - Gel-Permeation Chromatography (GPC)

Gel Permeation Chromatography (GPC) is a size exclusion cleanup procedure recommended for the elimination of lipids, polymers, copolymers, proteins, natural resins and polymers, cellular components, and dispersed high-molecular weight compounds from sample extracts. GPC is appropriate for both polar and non-polar analytes, so it can be effectively used to cleanup extracts containing a broad range of target compounds.

At Caliber Analytical Services, sediment sample preparations are initiated using the Accelerated Solvent Extraction (ASE) technique. GPC is used to cleanup the resultant sample extracts prior to the analysis of semi-volatile organic compounds (EPA 8270C), including pesticides (EPA 8081A). The analysis for polycyclic aromatic hydrocarbons (PAHs) is performed using Gas Chromatograph /Mass Spectrometry (GC/MS) operated in both the scan and Selective Ion Monitoring (SIM) modes to maximize sensitivity.